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Updated: Aug 8, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Published on: January 7, 2017
Synthesis of a novel cyclic donor-acceptor conjugate for selective recognition of ATP
Prakash P Neelakandan1, Mahesh Hariharan, Danaboyina Ramaiah
1Photosciences and Photonics Division, Regional Research Laboratory (CSIR), Trivandrum 695019, India.
Abstract:
[reaction and structures: see text] Novel cyclophane 1 was synthesized, and its interactions with phosphate, adenosine, AMP, ADP, and ATP have been investigated. With addition of ATP, significant decrease in absorbance of 1 was observed, whereas other guest molecules showed negligible effect. The complex between 1 and ATP was confirmed through cyclic voltammetry and 1H NMR. The uniqueness of the system is that it complexes selectively with ATP in a cavity and involves synergistic effects of both electrostatic and pi-pi stacking interactions.
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