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Related Experiment Videos

Cholera toxin induced gene expression alterations.

Atabak R Royaee1, Chanaka Mendis, Rina Das

  • 1Department of Chemistry, Georgetown University, 37th, 654 Reiss Science Building, Washington, DC 20057, USA.

Molecular Immunology
|December 20, 2005
PubMed
Summary

Cholera toxin (CT) alters gene expression in human immune cells, impacting over 200 genes. These changes, particularly in immunity and inflammation, suggest CT

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Area of Science:

  • Immunology
  • Molecular Biology
  • Toxicology

Background:

  • Cholera toxin (CT) is known to induce cyclic AMP (cAMP), which regulates gene expression.
  • Limited information exists regarding specific gene expression alterations induced by CT.

Purpose of the Study:

  • To investigate gene expression changes in human lymphocytes and monocytes in response to CT.
  • To compare CT-induced gene expression profiles with those induced by adenylate cyclase modulators.

Main Methods:

  • Utilized cDNA microarrays to analyze the expression of 800 selected genes.
  • Exposed human lymphocytes and monocytes to varying concentrations and durations of CT.
  • Compared CT effects with forskolin (adenylate cyclase activator) and MDL-12 (inhibitor).

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Main Results:

  • Over 200 genes exhibited altered expression in CT-treated cells.
  • CT-responsive genes showed distinct patterns compared to forskolin and MDL-12.
  • Many affected genes are involved in immunity, inflammation, and oxidative stress.
  • CT induced a downregulation of Th1 markers and upregulation of Th2 markers.

Conclusions:

  • CT significantly alters gene expression in human immune cells.
  • CT's effects on gene expression are partly mediated through adenylate cyclase pathways.
  • CT influences immune responses, promoting a Th2-biased profile.