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Quantification of S-adenosyl methionine in microbial cell extracts
Jens Olaf Krömer1, Elmar Heinzle, Christoph Wittmann
1Biochemical Engineering, Saarland University, Saarbruecken, Germany.
Abstract:
A sensitive method for quantification of S-adenosyl methionine (SAM) in microbial cell extracts was developed and applied to Corynebacterium glutamicum. The method is based on SAM being completely hydrolyzed into (18)O-homoserine when extracted in boiling H(2) (18)O and thus can be clearly distinguished by GC-MS analysis from naturally labeled homoserine present in the cell extract. Additional quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination of both metabolites.
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