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Titration force microscopy on supported lipid bilayers.

Sergi Garcia-Manyes1, Pau Gorostiza, Fausto Sanz

  • 1Department of Physical Chemistry, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain.

Analytical Chemistry
|December 31, 2005
PubMed
Summary
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Chemically modified atomic force microscopy (AFM) probes act as nanosensors to measure surface charges on lipid bilayers. This technique allows for nanoscale chemical titrations, revealing surface pKa values through unique adhesion force peaks.

Area of Science:

  • Biophysics
  • Surface Chemistry
  • Analytical Chemistry

Background:

  • Atomic force microscopy (AFM) is a powerful tool for nanoscale imaging and force measurements.
  • Supported planar lipid bilayers are crucial models for biological membranes.
  • Understanding surface charge and pKa is vital for membrane interactions.

Purpose of the Study:

  • To develop a novel method for measuring surface charges of lipid bilayers using AFM.
  • To perform nanoscale chemical titrations on surfaces.
  • To determine the pKa of lipid bilayer surfaces and AFM probes.

Main Methods:

  • Utilizing chemically modified AFM probes in force spectroscopy mode.
  • Performing surface-based analytical chemistry titrations with weak acids attached to AFM tips.

Related Experiment Videos

  • Analyzing approaching and retracting force curves to study electrostatic interactions and adhesion forces.

    • Main Results:

    • AFM probes successfully measured surface charges of lipid bilayers with high sensitivity.
    • Electrostatic interactions significantly influence the force plots between the AFM tip and the membrane.
    • Adhesion force versus pH plots showed non-sigmoidal shapes with peaks attributed to hydrogen bonds and revealing pKa values.

    Conclusions:

    • Chemically modified AFM probes function as effective "nanosensors" for surface charge analysis.
    • This method enables nanoscale titrations, providing insights into surface chemistry.
    • The technique can determine the pKa of both the lipid bilayer surface and the AFM probe.