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[In vitro inhibitory effect of 23-HBA on angiogenesis]
Meng-jun Jiang1, Min Yang, Yao-yuan Zhou
1State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China. jmj16888@yahoo.com.cn
Aim:
To study the inhibitory effect of 23-HBA on angiogenesis in vitro.
Methods:
The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining.
Results:
The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA.
Conclusion:
23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
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