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Updated: Jul 9, 2026

Electroeluting DNA Fragments
Published on: September 5, 2010
DNA hole transport on an electrode: application to effective photoelectrochemical SNP typing
Akimitsu Okamoto1, Taku Kamei, Isao Saito
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan. okamoto@sbchem.kyoto-u.ac.jp
Abstract:
A useful feature of DNA is that long-range hole transport through DNA is readily achieved. Photostimulated long-range hole transport through DNA has prospective use in the development of a conceptually new electrochemical single-nucleotide polymorphism (SNP) typing method for use as a versatile platform for gene diagnostics and pharmacogenetics. We have applied artificial DNAs designed for photostimulated long-range hole transport through DNA to SNP typing. By hybridizing photosensitizer-equipped DNA probes, immobilized on gold working electrodes, with a target DNA strand containing an SNP site, we observed a cathodic photocurrent, which markedly changed depending on the nature of the base at the specific site. The use of a combination of hole-transporting bases constitutes a very powerful method for a single-step electrochemical assay applicable to SNP typing of all types of sequences.
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