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Related Experiment Videos

The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends.

Stefan Westermann1, Hong-Wei Wang, Agustin Avila-Sakar

  • 1Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720-3202, USA.

Nature
|January 18, 2006
PubMed
Summary
This summary is machine-generated.

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The Dam1 complex in budding yeast forms a ring that moves along depolymerizing microtubules, facilitating chromosome segregation during cell division. This molecular ring translates microtubule depolymerization force into movement, crucial for accurate chromosome movement.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Chromosomes segregate during mitosis via kinetochore-microtubule interactions.
  • The molecular machinery coupling microtubule depolymerization to chromosome movement in anaphase remains largely unknown.

Purpose of the Study:

  • To elucidate the molecular mechanism of chromosome-microtubule coupling during mitosis.
  • To characterize the function and structure of the Dam1 complex in chromosome segregation.

Main Methods:

  • Real-time, two-colour fluorescence microscopy assay to observe complex dynamics.
  • Electron microscopy for high-resolution structural analysis of the Dam1 complex.

Main Results:

  • The ten-protein Dam1 complex forms a ring that moves processively along depolymerizing microtubules.

Related Experiment Videos

  • Structural analysis revealed a 16-fold symmetry of the Dam1 ring, inconsistent with direct registry to microtubule's 13-fold symmetry.
  • This suggests a sliding mechanism mediated by an electrostatic interface.

    • Conclusions:

    • The Dam1 complex acts as a molecular device translating microtubule depolymerization into force for chromosome movement.
    • This mechanism is critical for accurate chromosome segregation during anaphase.
    • The Dam1 complex's structure facilitates efficient force transduction along the microtubule lattice.