Related Experiment Video
Updated: Aug 13, 2026

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
Published on: March 28, 2012
[Apoptosis and its molecular mechanism in vestibular hair cell after gentamycin toxicity]
Xuejun Jiang1, Wei Li, Hongrui Zang
1Department of Otolaryngology, Affiliated First Hospital of China Medical College, Shenyang, 110001, China. djiangxj@163.com
Objective:
To investigate the character of damaged vestibular hair cells caused by gentamycin, and to identify whether apoptosis and phosphorylation of JNK occurs in the damaged cells.
Method:
Healthy guinea pigs were divided into experimental group and control group randomly with 15 in each group, animals in experimental and control group received systemic injection of gentamycin [100 mg/(kg x d)] and saline respectively for 7 consequent days. All the animals were sacrificed on the 8th day, the crista vestibular of each were observed by preparation and frozen dissection. The apoptosis of hair cell were detected by TUNEL method and phosphorylation of JNK were detected by immunochemistry.
Result:
The lesion and apoptosis of hair cells could be seen in the experimental group,and the phosphorylation could be observed too; the hair cells of control group showed no sign of apoptosis or phosphorylation of JNK.
Conclusion:
Gentamycin cause vestibular hair lesion by inducing apoptosis and activation of JNK plays an important role in the procedure of apoptosis.
Related Concept Videos
Hair Cells
Unrenewable Cells
Photoreceptors
The retina is composed of several layers and contains specialized cells called photoreceptors. The photoreceptors (rods and cones) change their membrane potential when stimulated by light energy. There are two types of photoreceptors—rods and cones—which differ in the shape of their outer...
Cellular Injury V: Apoptosis and Autophagy

