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Updated: Aug 13, 2026
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
Published on: August 29, 2016
[Differentiation of bone marrow mesenchymal stem cells into hepatocytes in hepatectomized mouse]
Xiongzhi Wu1, Liansan Zhao, Qian Xu
1National Key Laboratory of Biotherapy, Infectious Diseases Center of West China Hospital of Sichuan University, Chengdu 610041, China.
Abstract:
CD44+ CD29+ and CD44- CD29+ bone marrow mesenchymal stem cells (BMSCs) were isolated from bone marrow of BALB/C male donor mouse by adherence selecting in DMEM media with low glucose and 20% fetal bovine serum. Then P5 cells were injected into the liver remnants of hepatectomined female mouse. Simultaneous assays were performed on the injected liver lobe and other liver lobe for detecting the Y-chromosome by fluorescence in situ hybridization and for defecting the albumin or cytokeratin 18 (CK18) by fluorescence immunoassays. Both Y chromosome and albumin (or CK18) positive cell could be detected at 5 days and 14 days after transplantation. At 14 days after transplantation, the liver weight in transplanted mouse was higher than that in model mouse. These results suggest that BMSCs could be induced to differentiate into hepatocytes and participate in the regeneration of liver.
