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An ELISA for tissue factor using monoclonal antibodies.
S Albrecht1, T Luther, H Grossmann
1Institute of Pathology, Medical Academy Dresden, Germany.
Summary
Researchers developed a new assay to measure tissue factor (TF) expression. This method accurately quantifies TF on cells, aiding studies on inflammation, immunity, and cancer, and is preferable to clotting assays for certain applications.
Area of Science:
- Biochemistry
- Immunology
- Oncology
Background:
- Tissue factor (TF) is a receptor crucial for blood coagulation.
- Normally absent in vasculature, TF is induced on monocytes and endothelial cells during inflammation and immune responses.
- TF expression on tumor cells suggests a role in cancer growth and metastasis.
Purpose of the Study:
- To develop tools for studying TF's role in biological processes.
- To create a sensitive assay for quantifying TF expression on cells.
Main Methods:
- Development of monoclonal antibodies against human tissue factor apoprotein.
- Characterization of antibodies via neutralization of procoagulant activity and immunoblotting.
- Creation of a sandwich ELISA using monoclonal antibodies for rapid TF quantitation.
Main Results:
- The sandwich ELISA demonstrated high sensitivity for TF detection.
- Assay results correlated well with functional clotting assays.
- ELISA method is advantageous as it is unaffected by other coagulation factors or inhibitors.
Conclusions:
- Monoclonal antibodies and a sandwich ELISA provide a sensitive method for monitoring cellular TF expression.
- This assay facilitates research into TF's role in inflammation, immunity, and cancer.
- The ELISA offers a preferred alternative to clotting assays for specific TF quantitation applications.