Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aqueous compatible polymers in bionanotechnology.

S R Carter1, S Rimmer

  • 1Department of Chemistry (Polymer Centre), University of Sheffield, Sheffield, South Yorkshire, S3 7HF, UK. s.r.carter@sheffield.ac.uk

IEE Proceedings. Nanobiotechnology
|January 31, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing short-term mortality between people with and without HIV admitted to the intensive care unit: A single-centre matched cohort study (2000-2019).

HIV medicine·2024
Same author

Development of a novel micro-bead force spectroscopy approach to measure the ability of a thermo-active polymer to remove bacteria from a corneal model.

Scientific reports·2021
Same author

User testing to examine patient understanding of pharmacy generated medication labels.

Patient education and counseling·2020
Same author

Epithelioid sarcomas: How important is loco-regional control?

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2017
Same author

What is the morbidity of a non-invasive growing prosthesis?

The bone & joint journal·2016
Same author

Angle selective backscattered electron contrast in the low-voltage scanning electron microscope: Simulation and experiment for polymers.

Ultramicroscopy·2016
Same journal

Dynamics of rotating paramagnetic particles simulated by lattice Boltzmann and particle dynamics methods.

IEE proceedings. Nanobiotechnology·2006
Same journal

Absorption detection of enzymatic reaction using optical microfluidics based intermittent flow microreactor system.

IEE proceedings. Nanobiotechnology·2006
Same journal

Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core waveguide.

IEE proceedings. Nanobiotechnology·2006
Same journal

Application of nano-indenter for investigation of the properties of the elytra cuticle of the dung beetle (Copris ochus Motschulsky).

IEE proceedings. Nanobiotechnology·2006
Same journal

Characterisation of pore structures in nanoporous materials for advanced bionanotechnology.

IEE proceedings. Nanobiotechnology·2006
Same journal

Freestanding polyelectrolyte multilayers as functional and construction elements.

IEE proceedings. Nanobiotechnology·2006
See all related articles

Researchers developed novel core-shell molecularly imprinted particles (CS-MIPs) for specific molecular recognition. These nanoparticles and functional polymers show potential for applications in complex biological matrices and temperature-sensitive protein purification.

Area of Science:

  • Nanotechnology
  • Polymer Chemistry
  • Biomaterials

Background:

  • Molecularly imprinted polymers (MIPs) offer selective recognition capabilities.
  • Developing nanomaterials for specific molecular binding is crucial for diagnostics and therapeutics.
  • Aqueous-compatible polymers are needed for biological applications.

Purpose of the Study:

  • To synthesize and characterize core-shell molecularly imprinted particles (CS-MIPs) for caffeine recognition.
  • To synthesize water-soluble, highly-branched imidazole end-chain functionalized polymers.
  • To explore the potential of these nanomaterials in molecular recognition and protein precipitation.

Main Methods:

  • Emulsion polymerization was used to create CS-MIPs with caffeine and theophylline as templates.

Related Experiment Videos

  • Radiolabeling studies confirmed template localization on CS-MIPs.
  • Reversible addition-fragmentation chain transfer (RAFT) polymerization synthesized functional polymers.
  • Scatchard binding analysis assessed molecular recognition specificity.
  • Affinity precipitation was used for protein purification.

    • Main Results:

    • Caffeine-imprinted CS-MIPs demonstrated specific binding to caffeine, showing a biphasic Scatchard curve.
    • Theophylline-imprinted CS-MIPs exhibited monophasic binding.
    • Synthesized polymers displayed lower critical solution temperatures below ambient.
    • These polymers proved effective in the affinity precipitation of temperature-sensitive proteins like BRCA1.

    Conclusions:

    • CS-MIPs can be designed for selective molecular recognition of small molecules in biological systems.
    • Functionalized nanodimensions polymers with tunable LCST are effective for temperature-sensitive protein purification.
    • These diverse aqueous-compatible polymers hold promise for nanoscale molecular recognition applications.