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Fish-bone peptide increases calcium solubility and bioavailability in ovariectomised rats
Won-Kyo Jung1, Bae-Jin Lee, Se-Kwon Kim
1Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
The British Journal of Nutrition
|January 31, 2006
Summary
Fish-bone peptides (FBP) enhance calcium absorption and bone health. Supplementation in ovariectomized rats improved bone mineral density and strength, demonstrating FBP
Area of Science:
- Biochemistry
- Nutrition Science
- Biotechnology
Background:
- Calcium (Ca) deficiency impacts bone health, particularly in post-menopausal women.
- Fish-bone peptides (FBP) are protein fragments derived from fish bones.
- Investigating novel sources for calcium bioavailability enhancers is crucial.
Purpose of the Study:
- To isolate and characterize fish-bone peptides (FBP) with high affinity for calcium.
- To evaluate the in vitro and in vivo effects of FBP II on calcium bioavailability and bone metabolism.
- To assess the potential of FBP as a dietary supplement for preventing calcium deficiency.
Main Methods:
- Hydroxyapatite affinity chromatography for FBP isolation.
- Ultramembrane filtration for FBP II fractionation based on molecular weight (5.0-1.0 kDa).
- In vitro studies on calcium salt precipitation and in vivo studies using ovariectomized rats fed a low-calcium diet.
Main Results:
- FBP II inhibited insoluble calcium salt formation in vitro.
- FBP II supplementation increased calcium retention and decreased bone mineral loss in ovariectomized rats.
- FBP II significantly improved femoral total calcium, bone mineral density, and strength, comparable to casein phosphopeptide.
Conclusions:
- Fish-bone peptides (FBP II) enhance calcium bioavailability.
- FBP II demonstrates beneficial effects in preventing calcium deficiency and improving bone health.
- Fish-meal derived peptides show promise as a functional food ingredient for bone health.