Related Experiment Video
Updated: Aug 11, 2026

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
Published on: March 12, 2021
[CDNA microarray on differentially expressed genes of adipose tissue in two breeds chicken]
Hong-Bao Wang1, Qi-Gui Wang, Hui Li
1The College of Animal Science and Technology, Northeast Agriculture University, Harbin 150030, China.
Abstract:
cDNA microarray containing 9024 cDNAs was used to construct gene expression profile in order to screen differentially expressed genes of adipose tissue between broiler and Bai' er and investigate the molecular mechanism related with body fatness traits between the two breeds. Sixty seven differentially expressed genes, being involved in fat metabolism, energy metabolism, cytoskeleton, transcription and splicing factor, protein synthesis and degradation, were screened out. Furthermore, some genes that had no annotation in GenBank were screened out, they were presumed to be unknown new genes. The roles that they may play in chicken fat metabolism need clarify later.

