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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
Published on: December 9, 2016
Single amino-acid InDel variants generated by alternative tandem splice-donor and -acceptor selection
Chun-Hung Lai1, Ling-Yueh Hu, Wen-chang Lin
1Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, ROC.
Abstract:
We have investigated putative single amino-acid InDel variants with human ESTs. Examination of the formation process for single amino-acid InDel variants indicates a possible splicing mechanism in addition to the genomic insertion/deletion events as would be expected. The wobble-splicing transcripts were often generated around the intron-exon boundaries by selecting an alternative neighboring splice signal sequence, in particular the tandem agNAG or GTNgt sequence at the splice-acceptor or -donor site, thus creating single amino-acid InDel isoforms. Another category of variants was identified with one altered amino-acid plus one amino-acid InDel, under divergent coding-frame usage. We demonstrate that such minute distance of splice site choice generates an even greater level of transcriptome diversity, and suggest that non-functional synonymous or intronic SNPs could be converted to functionally significant InDel alterations through this process. This subtle alteration in mRNA and protein-coding sequence may elicit a great impact upon human genome and proteome diversity.
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