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Herpesvirus saimiri-based gene delivery vectors
Rhoswyn A Griffiths1, James R Boyne, Adrian Whitehouse
1Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, LS2 9JT, UK.
Current Gene Therapy
|February 16, 2006
Summary
Herpesvirus saimiri (HVS) shows potential as a gene delivery vector due to its ability to deliver large DNA amounts and establish persistent infections. HVS vectors offer stable gene expression in dividing cells, aiding genetic disease treatments.
Area of Science:
- Virology
- Gene Therapy
- Molecular Biology
Background:
- Herpesviruses exhibit traits suitable for gene delivery vectors, including large DNA packaging capacity.
- They establish persistent, lifelong infections with viral genomes remaining as circular, non-integrated episomes.
Purpose of the Study:
- To review the assessment of Herpesvirus saimiri (HVS)-based vectors.
- To highlight advancements and potential applications in treating genetic diseases.
Main Methods:
- Assessment of HVS-based vectors in in vitro studies.
- Evaluation of HVS-based vectors in in vivo studies.
Main Results:
- HVS vectors efficiently infect various human cell lines and primary cultures.
- Viral genomes persist as high-copy number, circular episomes, segregating to progeny cells.
- HVS vectors enable stable transduction of dividing cells and sustained gene expression.
Conclusions:
- HVS vectors combine artificial chromosome characteristics with efficient delivery.
- They offer a promising system for sustained heterologous gene expression in dividing cell populations.
- HVS vectors present potential applications for genetic disease therapies.