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Gene expression analysis using filter cDNA microarrays

Peter Simpson1, Chris Jones, Alan Mackay

  • 1The Breakthrough Toby Robins Breast Research Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.

Methods in Molecular Medicine
|February 24, 2006
PubMed

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DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...