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Updated: Aug 10, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in
1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.
Abstract:
In Fusarium verticillioides, the C-type cyclin Fcc1 is a global regulator of gene expression. In Saccharomyces cerevisiae and other organisms, C-type cyclins regulate the activity of specific cyclin-dependent kinases through physical association. We identified FCK1, a gene encoding a cyclin-dependent kinase in F. verticillioides. The Fck1 protein is predicted to contain a cyclin-binding motif and a serine-threonine protein kinase domain homologous to previously described cyclin-dependent kinases. Disruption of FCK1 resulted in pleiotropic morphological defects including reduced growth, conidiation, fumonisin B(1) (FB(1)) production, and enhanced pigmentation. Two-hybrid analysis indicated a strong physical interaction between Fcc1 and Fck1. This study presents the first description of the interaction between a C-type cyclin and a cyclin-dependent kinase in a filamentous fungus and provides new insight regarding a molecular mechanism that regulates aspects of maize kernel colonization by F. verticillioides.
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