Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse morphological phenotyping with magnetic resonance imaging.

X Josette Chen1

  • 1Department of Medical Biophysics, University of Toronto, Ontario, Canada.

Methods in Molecular Medicine
|March 2, 2006
PubMed
Summary

Magnetic resonance imaging (MRI) is increasingly used for mouse phenotyping to identify human disease models. This guide details essential animal handling for robust in vivo and postmortem imaging, including multi-mouse techniques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mouse phenotyping with MRI.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Trading off SNR and resolution in MR images.

NMR in biomedicine·2009
Same author

Optimization of the SNR-resolution tradeoff for registration of magnetic resonance images.

Human brain mapping·2007
Same author

MR technology for biological studies in mice.

NMR in biomedicine·2007
Same author

Mouse behavioral mutants have neuroimaging abnormalities.

Human brain mapping·2007
Same author

Tbx5-dependent rheostatic control of cardiac gene expression and morphogenesis.

Developmental biology·2006

Area of Science:

  • Biomedical imaging
  • Preclinical research
  • Mouse models

Background:

  • Magnetic resonance imaging (MRI) is a rapidly advancing field for mouse phenotyping.
  • Both MRI physicists and biologists are utilizing MRI to identify mouse models of human disease.

Purpose of the Study:

  • To provide detailed protocols for animal handling in routine and robust in vivo MRI.
  • To emphasize techniques for multiple-mouse imaging.
  • To describe perfusion-fixation methods for postmortem imaging of mice and specimens.

Main Methods:

  • In vivo animal handling for magnetic resonance imaging.
  • Multiple-mouse imaging techniques.
  • Perfusion-fixation protocols for ex vivo imaging.

Related Experiment Videos

Main Results:

  • Established protocols for consistent and reliable in vivo mouse MRI.
  • Demonstrated methods for efficient simultaneous imaging of multiple mice.
  • Detailed procedures for optimal postmortem specimen preparation.

Conclusions:

  • Standardized animal handling is crucial for robust in vivo mouse phenotyping using MRI.
  • Multi-mouse imaging enhances throughput for preclinical studies.
  • Perfusion-fixation techniques enable detailed postmortem analysis of mouse models.