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Updated: Aug 1, 2026
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
Published on: February 27, 2011
Mouse morphological phenotyping with magnetic resonance imaging
1Department of Medical Biophysics, University of Toronto, Ontario, Canada.
Abstract:
The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, with both MRI physicists and biologists starting to use MRI to identify mouse models of human disease. The purpose of this chapter is to provide details of the animal handling necessary for routine and robust in vivo imaging with particular emphasis on multiple-mouse imaging. In addition, techniques for perfusion-fixation for postmortem imaging of specimens and whole mice are given.
Insights
Magnetic resonance imaging (MRI) is increasingly used for mouse phenotyping to identify human disease models. This guide details essential animal handling for robust in vivo and postmortem imaging, including multi-mouse techniques.
Area of Science:
- Biomedical imaging
- Preclinical research
- Mouse models
Background:
- Magnetic resonance imaging (MRI) is a rapidly advancing field for mouse phenotyping.
- Both MRI physicists and biologists are utilizing MRI to identify mouse models of human disease.
Purpose of the Study:
- To provide detailed protocols for animal handling in routine and robust in vivo MRI.
- To emphasize techniques for multiple-mouse imaging.
- To describe perfusion-fixation methods for postmortem imaging of mice and specimens.
Main Methods:
- In vivo animal handling for magnetic resonance imaging.
- Multiple-mouse imaging techniques.
- Perfusion-fixation protocols for ex vivo imaging.
Main Results:
- Established protocols for consistent and reliable in vivo mouse MRI.
- Demonstrated methods for efficient simultaneous imaging of multiple mice.
- Detailed procedures for optimal postmortem specimen preparation.
Conclusions:
- Standardized animal handling is crucial for robust in vivo mouse phenotyping using MRI.
- Multi-mouse imaging enhances throughput for preclinical studies.
- Perfusion-fixation techniques enable detailed postmortem analysis of mouse models.
