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[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells]
Chun-hua Qie1, Bo Qin, Ai-long Huang
1Viral Institute for Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400010, China.
Aim:
To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.
Methods:
Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells. The expression of fusion gene encoding Hyper-IL-6 was detected by GFP and RT-PCR and immunocytochemistry.
Results:
The recombinant plasmid pIRES-HIL-6 was successfully constructed and expressed in mammalian cells.
Conclusion:
The success in construction and expression of human Hyper-IL-6 makes it possible to further study its biological functions.
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