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Published on: May 19, 2023
[Bioinformatics analysis of YZ-2 and preparation of polyclonal antibody]
Lin-quan Zang1, Peng-xin Qiu, Ru Xiao
1Department of Pharmacology, Pharmaceutical School, Guangdong Pharmaceutical University, Guangzhou 510006, China. zanglq@yahoo.com
Aim:
To analyze YZ-2, a novel protein associated with cerebral ischemia and prepares its polyclonal antibody.
Methods:
According to the bioinformatics analysis and prediction of the possible high structure, hydrophilicity and antigenicity of YZ-2, a 22-amino acid residue partial peptide of YZ-2 was synthesized. The synthesized peptide was then used to immunize. And the properties of anti-YZ2 were analyzed by ELISA and Western blot.
Results:
The hydrophilicity and antigenicity were predicted by methods of bioinformatics. The polyclonal antibody of YZ-2 was successfully obtained and its specificity and sensitivity were conformed by ELISA and Western blot.
Conclusion:
By the bioinformatics analysis and prediction, the hydrophilicity and antigenicity of YZ2 were analyzed. The antibody of YZ-2 was successfully obtained.
