Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A fluorescent nanosensor for apoptotic cells.

Luisa Quinti1, Ralph Weissleder, Ching-Hsuan Tung

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, 02129, USA.

Nano Letters
|March 9, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Intelligent catalase-coated MnO<sub>2</sub> nanoparticles with programmed oxygen supply and glutathione depletion for enhanced photodynamic therapy.

Journal of materials chemistry. B·2026
Same author

Tumor-Responsive Fluorogenic Spray for Image-Guided Cytoreduction of Disseminated Ovarian Tumors.

Bioconjugate chemistry·2025
Same author

The gain-of-function TREM2-T96K mutation increases risk for Alzheimer's disease by impairing microglial function.

Neuron·2025
Same author

Iron-Doxorubicin Hyaluronan Nanogel as an Effective Targeted Chemotherapy with Modulated Cardiotoxicity.

Biomacromolecules·2025
Same author

Hijacking the hyaluronan assisted iron endocytosis to promote the ferroptosis in anticancer photodynamic therapy.

Carbohydrate polymers·2025
Same author

Integrative pathway analysis across humans and 3D cellular models identifies the p38 MAPK-MK2 axis as a therapeutic target for Alzheimer's disease.

Neuron·2024
Same journal

Intrinsic Superconducting Gap in Bilayer KCa<sub>2</sub>Fe<sub>4</sub>As<sub>4</sub>F<sub>2</sub> and Decoupled Monolayer FeAs.

Nano letters·2026
Same journal

Programmable Hydrogen-Assisted Chemical Vapor Deposition Growth and Bipolar Transport in Two-Dimensional MoO<sub>2</sub> Nanoflakes.

Nano letters·2026
Same journal

A Curvature-Modulated Strategy for Single-Atom Catalysts toward Reciprocal Regulation in Li-S Batteries.

Nano letters·2026
Same journal

Vacuum Pyrolysis Engineered CoSb/C Scaffold for Sodium Metal Anodes with Sodiophilic and Superionic Interphase.

Nano letters·2026
Same journal

Hexagonal SiGe Quantum Dots in Nanowires.

Nano letters·2026
Same journal

Monolithic Axial InGaAs Quantum Dot Emitters in GaAs-Based Nanowires via Sb-Mediated Facet Engineering.

Nano letters·2026
See all related articles

Researchers developed novel nanoparticles that detect apoptotic cells by binding to phosphatidylserine. This technology enables accurate measurement of apoptosis induction, crucial for biomedical applications and in vivo imaging.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Apoptosis, or programmed cell death, is a critical biological process.
  • Detecting apoptotic cells is vital for understanding diseases and evaluating treatments.
  • Current methods for apoptosis detection have limitations.

Purpose of the Study:

  • To design and synthesize a novel biocompatible nanoparticle for sensing phosphatidylserine on apoptotic cells.
  • To functionalize magnetofluorescent nanoparticles with synthetic phosphatidylserine binding ligands.
  • To evaluate the efficacy of these nanoparticles in detecting and quantifying apoptotic cells.

Main Methods:

  • Conjugation of synthetic artificial phosphatidylserine binding ligands onto magnetofluorescent nanoparticles in a multivalent manner.

Related Experiment Videos

  • Binding assays to confirm nanoparticle interaction with apoptotic cells.
  • Correlation studies with traditional microscopy-based annexin V staining.
  • Flow cytometry (FACS) analysis to quantify therapeutic apoptosis induction.

    • Main Results:

    • The surface-functionalized nanoparticles demonstrated specific binding to apoptotic cells.
    • A strong correlation was observed between nanoparticle staining and annexin V staining.
    • Flow cytometry analysis using the nanoparticles effectively measured therapeutic apoptosis induction.

    Conclusions:

    • The developed magnetofluorescent nanoparticles are effective biosensors for detecting phosphatidylserine on apoptotic cells.
    • This nanomaterial offers a reliable method for quantifying apoptosis, correlating well with established techniques.
    • These nanoparticles hold significant promise for various biomedical applications, including in vivo imaging of apoptosis.