Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Second generation biosensors.

F W Scheller1, F Schubert, B Neumann

  • 1Academy of Sciences, Central Institute of Molecular Biology, Berlin-Buch, FRG.

Biosensors & Bioelectronics
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure control at the first dark-field chest radiography demonstrator system.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

Finite element fracture load analysis and dark-field X-ray imaging of osteoporotic and healthy vertebrae in human lumbar spine specimens.

BMC musculoskeletal disorders·2025
Same author

Rapid metabolism fosters microbial survival in the deep, hot subseafloor biosphere.

Nature communications·2022
Same author

Scaling behavior of the reorientation kinetics of block copolymers exposed to electric fields.

Soft matter·2020
Same author

Corrigendum: Violent Behavior During Psychiatric Inpatient Treatment in a German Prison Hospital.

Frontiers in psychiatry·2020
Same author

Violent Behavior During Psychiatric Inpatient Treatment in a German Prison Hospital.

Frontiers in psychiatry·2019
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
Same journal

Synergistic mode-field pre-expansion and geometric compression in hetero-structured microfibers for ultrasensitive glucose sensing.

Biosensors & bioelectronics·2026
Same journal

An amplification-free dual-readout biosensor integrating colorimetry and single-particle counting for ultrasensitive miRNA detection in esophageal cancer.

Biosensors & bioelectronics·2026
Same journal

An all-in-one microfluidic system via data-driven design for on-site genotyping of genetically modified foods.

Biosensors & bioelectronics·2026
See all related articles

This study presents advanced glucose sensors using enzyme-membrane electrodes. Innovations include improved analytical quality, interference suppression, and reagentless measurement for enhanced glucose determination.

Area of Science:

  • Biomedical Engineering
  • Electrochemistry
  • Biosensors

Background:

  • Enzyme-membrane electrodes with glucose oxidase are standard for diluted sample analysis.
  • Fast-responding glucose sensors utilize porous enzyme layers on thin metal electrodes.
  • Second-generation sensors co-immobilize auxiliary enzymes/co-reactants to enhance analytical quality.

Purpose of the Study:

  • To develop highly sensitive and reliable glucose sensors.
  • To improve analytical quality and simplify sensor performance.
  • To enable reagentless glucose measurement and suppress interferences.

Main Methods:

  • Fabrication of fast-responding glucose sensors using porous enzyme layers on thin metal electrodes.
  • Co-immobilization of glucose oxidase/peroxidase complex to suppress oxidizable interferences.

Related Experiment Videos

  • Utilizing a glucose oxidase/peroxidase layer-covered fluoride FET for potentiometric determination.
  • Incorporating NAD bound to PEG in glucose dehydrogenase layer for reagentless sensing.

    • Main Results:

    • Achieved extremely fast response times in glucose sensors.
    • Suppressed oxidizable interferences using a glucose oxidase/peroxidase complex at low potentials.
    • Demonstrated insensitivity to pH and buffer capacity fluctuations with fluoride FET sensors.
    • Increased sensitivity by several orders of magnitude through enzymatic analyte recycling/intermediate accumulation.

    Conclusions:

    • Developed advanced enzyme-membrane electrodes for superior glucose sensing.
    • Innovations enable enhanced analytical quality, interference reduction, and reagentless operation.
    • These glucose sensors offer significant improvements for laboratory analysis and diagnostics.