A novel heterobifunctional linker for facile access to bioconjugates

Area of Science:

• Chemical Biology
• Synthetic Chemistry
• Bioconjugation

Background:

• Developing versatile cross-linking reagents is crucial for constructing complex biomolecular assemblies.
• Existing methods may lack efficiency or specificity for certain biomolecule pairings.

Purpose of the Study:

• To synthesize and characterize a novel heterobifunctional linker molecule.
• To demonstrate the utility of this linker in creating conjugates between different biomolecules.

Main Methods:

• Synthesis of a heterobifunctional linker containing a nitropyridyl disulfide (Npys) group and an aminooxy group.
• Utilizing the linker to conjugate a peptide and an oligonucleotide via disulfide and oxime linkages.

Main Results:

• A convenient and efficient synthesis of the novel heterobifunctional linker was achieved.
• The linker successfully facilitated the formation of a peptide-oligonucleotide conjugate.
• The linker demonstrated its capability for cross-linking distinct biomolecules.

Conclusions:

• The novel heterobifunctional linker provides a valuable tool for bioconjugation.
• This linker enables efficient cross-coupling of functionalized biomolecules, such as peptides and oligonucleotides.
• The developed linker expands the toolkit for creating sophisticated biomolecular constructs.