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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
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[Plaque characteristics of herpes mamillitis virus (HMV)]

M Dilovski

    Veterinarno-Meditsinski Nauki
    |January 1, 1975
    PubMed

    Insights

    Bovine herpes mammillitis virus (BHV-1) forms uniform plaques. The plaque reduction test is precise for quantifying BHV-1, with starch and agarose yielding optimal results.

    Area of Science:

    • Virology
    • Veterinary Medicine
    • Cell Biology

    Context:

    • Bovine herpes mammillitis virus (BHV-1) is a significant pathogen in cattle.
    • Understanding viral plaque formation is crucial for accurate diagnostic assays.
    • Standardization of plaque assays is essential for reliable virus quantification.

    Purpose:

    • To characterize the plaque morphology of bovine herpes mammillitis virus (BHV-1).
    • To evaluate the plaque reduction test's precision and specificity for BHV-1.
    • To compare different overlay substances for optimal BHV-1 plaque assay performance.

    Summary:

    • BHV-1 produces homogeneous plaques, typically round or slightly irregular, 2-3 mm in diameter with semitranslucent centers.
    • Microscopic examination reveals necrotic centers and surrounding cellular changes.
    • Plaque number correlates directly with virus concentration, validating the plaque reduction test's utility.
    • Hydrolysed starch and agarose are superior overlay agents for BHV-1 plaque studies compared to agar or carboxylmethylcellulose.

    Impact:

    • Provides detailed morphological characterization of BHV-1 plaques.
    • Establishes the plaque reduction neutralization test as a precise and specific method for BHV-1 quantification.
    • Recommends optimal overlay substances (starch and agarose) for improved BHV-1 plaque assays in research and diagnostics.

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