[Experiments to identify Mycoplasma isolates from cell cultures]

Identified were various species of Mycoplasma organisms isolated from three continuous cell lines--BHK-21, SPZF, and TT. On the base of cultural, biochemical, and serological investigations the isolates were defined as M. bovirhinis, M. arginini, and A. laidlawii. The bovine sera, used to culture the cell lines were shown to be the basic source of contamination with Mycoplasma organisms. This made it necessary to carry out a preliminary study on each batch of serum for Mycoplasma contamination prior to its use for laboratory and productional needs.