Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Practical assay issues with the PERT/PBRT assay: a highly sensitive reverse transcriptase assay.

A Chang1, S Dusing

  • 1Biologics Testing, BioReliance, Invitrogen Bioservices, Rockville, MD 20850, USA. achang@bioreliance.com

Developments in Biologicals
|March 29, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual environment shapes the social dynamics of coloration and competition in an African Cichlid Fish.

The Journal of experimental biology·2026
Same author

Framework for AI integration into the undergraduate medical curricula: a scoping review.

BMC medical education·2026
Same author

General health survey in patients with small abdominal aortic aneurysm.

Frontiers in cardiovascular medicine·2025
Same author

Application of machine learning algorithms to predict osteoporosis in postmenopausal women with type 2 diabetes mellitus.

Journal of endocrinological investigation·2023
Same author

Novel role of estrogen receptor-α on regulating chondrocyte phenotype and response to mechanical loading.

Osteoarthritis and cartilage·2021
Same author

Discontinuation Patterns and Cost Avoidance of a Pharmacist-Driven Methicillin-Resistant <i>Staphylococcus aureus</i> Nasal Polymerase Chain Reaction Testing Protocol for De-escalation of Empiric Vancomycin for Suspected Pneumonia.

Open forum infectious diseases·2021

PCR-based reverse transcriptase assays (PERT) offer highly sensitive retrovirus detection for product safety. This method enhances screening for contamination, quantifies viral load, and monitors retrovirus generation in cell lines.

Area of Science:

  • Biotechnology
  • Virology
  • Product Safety Testing

Background:

  • Retrovirus detection is crucial for ensuring the safety of biological products.
  • Conventional screening assays like electron microscopy and PCR have limitations in sensitivity.
  • Existing methods for detecting reverse transcriptase activity can be less sensitive than molecular assays.

Purpose of the Study:

  • To describe the application of highly sensitive PCR-based reverse transcriptase assays (PERT) for retrovirus detection.
  • To highlight the advantages of PERT assays over conventional methods in product safety testing.
  • To demonstrate the utility of PERT assays in various aspects of retrovirus testing.

Main Methods:

  • Utilized PCR-based reverse transcriptase assays (PERT) for enhanced sensitivity.

Related Experiment Videos

  • Compared PERT assay performance with conventional methods like electron microscopy and nucleotide incorporation assays.
  • Applied PERT assays to screen for adventitious retrovirus contamination.

    • Main Results:

    • PERT assays demonstrated approximately a million-fold increase in sensitivity compared to conventional nucleotide incorporation assays.
    • Successfully applied PERT assays for screening adventitious retrovirus contamination in biologicals.
    • Demonstrated the capability of PERT assays to detect and quantify endogenous viral particle load and monitor retrovirus generation.

    Conclusions:

    • PERT assays represent a significant advancement in sensitive retrovirus detection for product safety.
    • The high sensitivity of PERT assays makes them ideal for rigorous testing of biologicals.
    • PERT assays offer versatile applications in identifying and monitoring retroviral elements in cell lines and products.