The method of melanocytes isolation

To judge the therapeutic value and evaluate the mechanism of action of this specific transplantation procedure we have performed an isolation of the melanocytes in experiment on the hens. The black hens (n=5), 3-5 kg of body weight were used in experimental study. Hens were housed in standard laboratory conditions under 12-hr day-night cycles with provision of pelleted diet and water ad libitum. All hens received care according to institutional guidelines. Using a hand dermatome, a shave biopsy specimen of approximately 2 cm2 was taken from the hens' normally pigmented region under local anesthesia (lidocaine hydrochloride, 20 mg/mL). The wound was covered with a transparent occlusive bandage (4 cm x 4 cm) and secured with gauze and adhesive tape. The melanocytes suspension have been obtained by fermentative digestion.