High-performance liquid chromatographic identification of flavonoid monoglycosides from Prunus serotina ehrh

Five minor flavonoid monosides, glycosides of quercetin and kaempferol, together with three previously isolated compounds were identified cochromatographically in P. serotina Ehrh. leaves and flowers (inflorescences) using RP-HPLC and TLC techniques and finally determined as quercetin 3-O-alpha-L-arabinofuranoside (avicularin), 3-O-alpha-L-arabinopyranoside (guaijaverin), 3-O-beta-D-xylopyranoside (reynoutrin), 3-O-beta-D-glucopyranoside (isoquercitrin), 3-O-beta-D-galactopyranoside (hyperoside) followed by kaempferol 3-O-alpha-L-arabinofuranoside (juglanin), 3-O-beta-D-xylopyranoside and 3-O-beta-D-glucopyranoside (astragalin). Moreover, two further minor flavonols were isolated from the leaves, characterized by hydrolysis experiments, UV and 1H NMR spectroscopy, and identified finally as isorhamnetin 3-O-alpha-arabinofuranoside and isorhamnetin 3-O-beta-xylopyranoside, the rare natural products.