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Microfluidic system for planar patch clamp electrode arrays.

Xiaohui Li1, Kathryn G Klemic, Mark A Reed

  • 1Department of Electrical Engineering, Yale University, New Haven, Connecticut 06520, USA.

Nano Letters
|April 13, 2006
PubMed
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We developed a microfluidic system with disposable partitions for simultaneous patch clamp recordings. This system achieved gigaseal recordings from RBL-1 cells, enabling simultaneous whole-cell recordings.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Electrophysiology

Background:

  • Patch clamp electrophysiology is crucial for studying ion channel function.
  • Traditional patch clamp methods can be time-consuming and require specialized expertise.
  • Developing high-throughput, automated electrophysiology systems is an ongoing challenge.

Purpose of the Study:

  • To present a novel microfluidic system for simultaneous patch clamp recordings.
  • To integrate disposable cell interface partitions with microfluidic channels and electrodes.
  • To assess the system's efficacy in obtaining high-quality gigaseal and whole-cell recordings.

Main Methods:

  • A microfluidic system was fabricated using poly(dimethylsiloxane) (PDMS) with integrated isolation valves.

Related Experiment Videos

  • Disposable glass-supported PDMS partitions with a 2-micrometer aperture were developed.
  • Reversible sealing of partitions to the microfluidic system was achieved.
  • Microfabricated Ag/AgCl electrodes were incorporated for electrophysiological recordings.
  • RBL-1 cells were used for patch clamp experiments.

    • Main Results:

    • Gigaseal recordings were successfully obtained from RBL-1 cells with a 24% success rate.
    • Simultaneous whole-cell recordings were achieved using valve-isolated electrodes.
    • The system demonstrated the feasibility of disposable partitions for patch clamp applications.

    Conclusions:

    • The presented microfluidic system enables simultaneous patch clamp recordings.
    • Disposable cell interface partitions offer a promising approach for electrophysiology.
    • This technology has the potential to streamline ion channel research and drug discovery.