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The stem cells of a primordial germ cell-derived teratocarcinoma have the ability to form viable mouse chimeras
K Hanaoka1, M Hayasaka, T Noguchi
1Laboratory of Mammalian Developmental Biology, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Abstract:
A euploid testicular teratocarcinoma line, STT-3, has been established from a tumor spontaneously occurring in the testis of a 129/Sv-ter male. Developmental ability of the STT-3 stem cells was tested by injecting these cells into mouse blastocysts. The frequency and the extent of chimerism were examined in mid-gestational fetuses and in live-born mice. STT-3 stem cells form viable chimeras at a high rate and differentiate into normal tissues. This is the first reported testicular teratocarcinoma-derived stem line with a proven capacity to form viable chimeric mice upon injection into the blastocysts.