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Updated: Aug 5, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Detection of HCV antigen in the serum by immunodotting]
Hong-bo Shi1, Li Xie, De-zhuang Huang
1Beijing Municipal Hepatitis Institute, Beijing You'an Hospital, Capital University of Medical Sciences, Beijing 100054, China. shb780727@tom.com
Aim:
To set up a simple and rapid screening method of early detection for HCV infection.
Methods:
The HRP-anti-HCV monoclonal antibodies (mAbs) conjugate was prepared by anti-HCV-core and anti-HCV-NS3 mAb with high specificity. HCV antigens in the sera from hepatitis patients and normal persons were detected by immunodotting and compared by double antibody ELISA and RT-PCR.
Results:
The positive ratio for HCV antigen was 15.4% (8/52), 1.73% (19/1099) and 1.03% (1/97) in hepatitis C patients, hepatitis B patients and hepatitis NA-NE patients, respectively. The results for HCV antigen were negative in hepatitis A patients (0/81), hepatitis E patients (0/67) and normal persons (0/50). There was no significant difference among the immunodotting and double antibody ELISA and RT-PCR. There was definite correlation between the immunodotting and RT-PCR.
Conclusion:
This method, simple and rapid with high specificity, provides an effective way for the early diagnosis and routine detection of HCV.
