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Electrogenic sucrose transport in developing soybean cotyledons.

F T Lichtner1, R M Spanswick

  • 1Section of Botany, Genetics and Development, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.

Plant Physiology
|April 1, 1981
PubMed
Summary

Soybean cotyledons show a transient membrane potential change when sucrose is added, indicating carrier-mediated sugar transport. This process is dependent on proton gradients and sensitive to specific inhibitors.

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Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Membrane potential changes are crucial for cellular functions.
  • Sugar transport mechanisms in plants are vital for development and energy distribution.
  • Understanding carrier-mediated transport requires investigating proton gradients and protein interactions.

Purpose of the Study:

  • To investigate the electrophysiological response of soybean cotyledons to sucrose addition.
  • To elucidate the role of proton electrochemical gradients in sucrose transport.
  • To identify the mechanisms and inhibitors of sucrose carrier proteins.

Main Methods:

  • Electrophysiological techniques were used to measure membrane potential changes in excised soybean cotyledons.
  • Varying concentrations of sucrose and protons were applied to assess transport kinetics.

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  • The effects of proton gradient disruptors and sulfhydryl-modifying reagents were evaluated.

    • Main Results:

    • Sucrose addition induced a transient depolarization of the membrane potential.
    • The depolarization magnitude correlated with sucrose and proton concentrations, suggesting carrier mediation with a K(m) of approximately 10 mM for sucrose.
    • Disruption of proton electrochemical gradients abolished the depolarization.
    • Sulfhydryl-modifying reagents and p-Chloromercuribenzene sulfonate inhibited the electrogenic sugar transport, indicating interaction with the carrier protein or proton gradient.

    Conclusions:

    • Electrogenic sucrose transport in soybean cotyledons is mediated by a carrier protein.
    • This transport process is dependent on the proton electrochemical gradient across the plasma membrane.
    • Specific reagents, like p-Chloromercuribenzene sulfonate, can selectively inhibit this carrier-mediated sucrose uptake.