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Sucrose uptake by developing soybean cotyledons.

F T Lichtner1, R M Spanswick

  • 1Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.

Plant Physiology
|September 1, 1981
PubMed
Summary

Soybean cotyledons utilize a proton-coupled sucrose transporter for sugar uptake, which becomes saturated at high sucrose levels. This carrier-mediated process is crucial for sucrose accumulation in developing seeds.

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Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Developing soybean cotyledons accumulate sucrose, a critical process for seed development.
  • Understanding the mechanisms of sucrose transport is vital for improving crop yields.

Purpose of the Study:

  • To elucidate the mechanism of sucrose uptake in developing soybean cotyledons.
  • To characterize the kinetics and regulatory factors of sucrose transport.

Main Methods:

  • Analysis of sucrose uptake kinetics at varying concentrations.
  • Investigation of proton gradient dependence using metabolic inhibitors (NaN3, CCCP).
  • Assessment of sulfhydryl-modifying agents (NEM, PCMBS) and reducing agents (DTE) on transport.

Main Results:

  • Sucrose uptake exhibits biphasic kinetics: carrier-mediated at low concentrations (Km=8 mM) and linear at high concentrations.
  • Transport is dependent on proton electrochemical potential, inhibited by NaN3 and CCCP.
  • The saturable component is sensitive to sulfhydryl reagents, with differential effects of NEM and PCMBS.
  • Transport is pH-dependent, decreasing at higher external pH.

Conclusions:

  • A sucrose/proton cotransporter mechanism is proposed for soybean cotyledon cells.
  • Carrier-mediated transport is saturated in vivo, suggesting it may limit sucrose accumulation.
  • The identified transporter plays a key role in seed development and carbon allocation.

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