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Relationship between the expression of advanced glycation end-products (AGE) and the receptor for AGE (RAGE) mRNA in

Daisuke Suzuki1, Masao Toyoda, Naoyuki Yamamoto

  • 1Division of Nephrology and Metabolism, Department of Internal Medicine, School of Medicine, Tokai University, Isehara.

Internal Medicine (Tokyo, Japan)
|May 9, 2006
PubMed
Abstract

Insights

Advanced glycation end-products (AGE) and the receptor for advanced glycation end-products (RAGE) are linked to kidney damage in diabetic nephropathy. Increased AGE and RAGE expression correlates with mesangial expansion in DN.

Area of Science:

  • Nephrology
  • Diabetology
  • Molecular Biology

Background:

  • The receptor for advanced glycation end-products (RAGE) is a cellular receptor for advanced glycation end-products (AGEs).
  • Diabetic nephropathy (DN) is a serious complication of diabetes characterized by kidney damage.
  • The roles of AGEs and RAGE in the pathogenesis of DN require further elucidation.

Purpose of the Study:

  • To investigate the relationship between AGE protein and RAGE mRNA expression in renal tissues of patients with diabetic nephropathy (DN).
  • To examine the correlation between AGE and RAGE expression and the degree of mesangial expansion in DN.

Main Methods:

  • Renal biopsy tissues from 20 DN patients and 5 controls were analyzed.
  • Immunohistochemistry for AGE protein and in situ hybridization for RAGE mRNA were performed.
  • Glomerular mesangial expansion (%Mes) was quantified using an image analyzer.

Main Results:

  • AGE protein was detected in the mesangial matrix of DN kidneys but not in controls.
  • RAGE mRNA was expressed in glomerular cells in both DN and control samples.
  • %Mes significantly correlated with both AGE protein staining intensity and RAGE mRNA expression.

Conclusions:

  • Both AGEs and RAGE are associated with the development and progression of diabetic nephropathy.
  • These findings highlight the potential involvement of the AGE-RAGE pathway in DN pathogenesis.

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