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A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
[Determination of methadone in plasma using high pressure liquid chromatography methods]
G Misztal1, L Przyborowski, A Wojtczak
1Katedra Chemii Leków, Akademia Medyczna, Lublin.
Acta Poloniae Pharmaceutica
|January 1, 1991
Summary
This study presents a high-performance liquid chromatography method for quantifying methadone in plasma. The validated method demonstrates high recovery rates, suitable for clinical and forensic analysis.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
- Chromatographic Techniques
Context:
- Accurate quantification of methadone in biological matrices is crucial for therapeutic drug monitoring and forensic toxicology.
- Existing methods may have limitations in sensitivity, specificity, or sample throughput.
- Development of robust analytical techniques is essential for reliable methadone level determination.
Purpose:
- To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of methadone in human plasma.
- To establish a reliable and efficient analytical procedure for methadone quantification.
- To assess the method's performance characteristics, including accuracy, precision, and recovery.
Summary:
- Methadone in plasma was quantified using high-performance liquid chromatography (HPLC) with a LiChrosorb RP-18 column.
- Protein precipitation with methanol and diethyl ether extraction from alkalized plasma preceded analysis.
- Methadone was detected at 254 nm after ion-pairing with pentane-1-sulphonic acid in an acetonitrile-methanol-ammonium acetate mobile phase, with piritramide as an internal standard.
- The method achieved a determination range of 0.25-1.25 µg/cm³ with 88-93% recovery.
Impact:
- Provides a validated HPLC method for accurate methadone measurement in plasma.
- Supports therapeutic drug monitoring and pharmacokinetic studies of methadone.
- Offers a reliable tool for clinical and forensic laboratories involved in drug analysis.
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