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Updated: Aug 8, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Published on: September 8, 2010
The effect of gap length on double-strand break repair in Drosophila
Dena M Johnson-Schlitz1, William R Engels
1Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA.
Abstract:
When a double-strand break has a gap between the broken ends, the missing information can be restored through synthesis from a homologous template. Here we address the question of how long such a gap can be before this process fails. We measured the frequency of homologous repair in the Drosophila germ line following the creation of gaps of specific sizes ranging from 3.8 to 210 kb. We found that gaps of
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