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Rabbits generated from fibroblasts through nuclear transfer.

Shangang Li1, Xuejin Chen, Zhenfu Fang

  • 1Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine and Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, PR China.

Reproduction (Cambridge, England)
|June 1, 2006
PubMed
Summary
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Somatic cell nuclear transfer successfully cloned rabbits from adult fibroblasts. While early offspring died, three cloned rabbits survived to adulthood, proving the viability of this technique for mammalian cloning.

Area of Science:

  • Reproductive biology
  • Mammalian cloning
  • Genetic engineering

Background:

  • Somatic cell nuclear transfer (SCNT) is a key technology for genetic engineering and genome preservation.
  • Previous SCNT studies in rabbits primarily utilized cumulus cells for successful cloning.

Purpose of the Study:

  • To investigate the potential of cultured adult rabbit fibroblasts as donor cells for SCNT.
  • To assess the developmental capacity and survival rates of cloned rabbits derived from adult fibroblasts.

Main Methods:

  • Fibroblasts were isolated from adult rabbits and cultured.
  • Serum-starved and non-starved fibroblasts were used for nuclear transfer into enucleated oocytes.
  • Embryos were transferred to surrogate mothers, with outcomes monitored via natural birth and Caesarean section.

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Main Results:

  • Nuclear transfer using serum-starved fibroblasts yielded a higher preimplantation developmental rate.
  • Eight of 20 surrogate mothers carried pregnancies to term.
  • Three of five rabbits born via Caesarean section survived to healthy adulthood, confirmed genetically identical to the donor.

Conclusions:

  • Adult rabbit fibroblasts can support full-term development after SCNT.
  • SCNT using cultured adult fibroblasts is a viable method for rabbit cloning.
  • Caesarean section improved the survival rate of cloned rabbits.