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Primary structure of mouse tyrosine hydroxylase deduced from its cDNA
S Ichikawa1, T Sasaoka, T Nagatsu
1Department of Biochemistry, Nagoya University School of Medicine, Japan.
Biochemical and Biophysical Research Communications
|May 15, 1991
Abstract:
The cDNAs for tyrosine hydroxylase were cloned from a mouse brain cDNA library by plaque hybridization. Since the longest cDNA clone lacked approximately 150 bp sequence of its N-terminal region, additional 5' region was obtained using polymerase chain reaction. Nucleotide sequence determination of cDNAs revealed that mouse tyrosine hydroxylase m-RNA encodes 498 amino acids with a calculated molecular mass of 55,990. The amino acid sequence of mouse tyrosine hydroxylase is highly homologous to rat (97%) and human (92%) enzymes.