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Direct and quantitative analysis of chromatin accessibility by MIRECAL--a Micrococcus nuclease/real-time PCR

Nina Graffmann1, Simeon Santourlidis, Julia Christ

  • 1Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University, D-40225 Düsseldorf, Germany.

Analytical Biochemistry
|June 6, 2006
PubMed
Abstract

No abstract available in PubMed .

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Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...