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Updated: Jul 31, 2026
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Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
Published on: August 31, 2013
Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
Juan Sanchez-Weatherby1, Stacey Southall, Arthur Oubrie
1School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England. j.sanchez-weatherby@uea.ac.uk
Abstract:
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
