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Extended storage of platelets in SSP platelet additive solution.
V S Hornsey1, K McColl, O Drummond
1Products and Components R&D Group, National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK. valerie.hornsey@snbts.csa.scot.nhs.uk
Vox Sanguinis
|June 8, 2006
Summary
Pooled platelets stored in SSP+ additive solution maintained quality for 9 days. Extended storage led to metabolic changes and platelet degradation, indicating a need for preparation optimization.
Area of Science:
- Transfusion Medicine
- Hematology
- Biochemistry
Background:
- Platelet additive solutions (PAS) are used to extend platelet storage.
- SSP+ is a PAS used for platelet storage.
Purpose of the Study:
- To evaluate the in vitro quality of pooled random platelets stored in SSP+ additive solution for up to 19 days.
Main Methods:
- Platelet concentrates were prepared using buffy coat-derived platelets in 75% SSP+ and 25% plasma.
- Platelets were stored in polyolefin bags for 19 days.
- In vitro analyses were performed at various time points during storage.
Main Results:
- Platelet quality was maintained until day 9.
- Glucose depletion by day 12 was followed by metabolic shifts (decreased pCO2, increased pO2, cessation of lactate production).
- Platelet activation markers (P-selectin, RANTES) increased, and platelet lysis occurred by day 19.
Conclusions:
- SSP+ can be used for platelet storage for up to 9 days.
- Platelet preparation in SSP+ requires optimization.
- In vivo studies are needed to confirm these findings.