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Developing a small animal model of extracorporeal circulation.
Amy M Skrzypchak1, Judiann Miskulin, Robert H Bartlett
1Department of General Surgery, Extracorporeal Life Support Laboratory, University of Michigan Medical Center, Ann Arbor, MI 48109-0243, USA.
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|June 9, 2006
Summary
Researchers developed two rabbit models for testing blood-contacting devices in extracorporeal circulation (ECC). The arteriovenous (AV) model proved superior due to its simplicity and effectiveness in evaluating nonthrombogenic materials.
Area of Science:
- Biomedical Engineering
- Cardiovascular Research
- Medical Device Development
Background:
- Extracorporeal circulation (ECC) requires nonthrombogenic materials to prevent blood clotting.
- Efficient small animal models are crucial for testing the biocompatibility of medical devices.
- Previous venovenous (VV) models faced technical challenges.
Purpose of the Study:
- To compare a novel arteriovenous (AV) rabbit model with a venovenous (VV) model for evaluating nonthrombogenic surfaces in ECC.
- To assess the efficacy of these models in detecting thrombogenicity.
- To determine the suitability of the AV model for filter or oxygenator simulation.
Main Methods:
- Two extracorporeal circulation models were established in anesthetized rabbits: a VV model and an AV model.
- Both models utilized PVC tubing circuits with specific catheter sizes for vascular access.
- Blood gases, platelet counts, and fibrinogen levels were monitored hourly over 4-hour study periods.
Main Results:
- Both VV and AV ECC models demonstrated significant platelet consumption, mirroring clinical observations.
- The AV model showed comparable results in platelet consumption, irrespective of an added chamber surface area.
- The AV model proved simpler to implement and manage compared to the VV model.
Conclusions:
- The arteriovenous (AV) rabbit model is a superior, simplified alternative to the venovenous (VV) model for nonthrombogenic surface testing in extracorporeal circulation.
- Both models effectively identify thrombogenic potential in medical devices.
- The AV model's design facilitates the simulation of oxygenators or filters, enhancing its utility in device development.