Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent aromatic platforms for cell patterning.

Jesus M de la Fuente1, Abhay Andar, Nikolaj Gadegaard

  • 1Centre for Cell Engineering, Institute of Biomedical and Life Science, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK. lafuente@iiq.csic.es

Langmuir : the ACS Journal of Surfaces and Colloids
|June 14, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface-driven endocrine activity of nanoplastics: polymer- and size-dependent estrogen and androgen receptors modulation without steroidogenesis perturbation.

Particle and fibre toxicology·2026
Same author

Metasurface analogues of molecular diastereomers from hierarchical multiscale chiral interactions with biomolecules.

Nature communications·2026
Same author

Transcriptomic and morphological characterization reveals distinct regeneration kinetics in immediate versus delayed nerve repair.

Neural regeneration research·2026
Same author

A guide to using embedded ethics in human stem-cell-based embryo model research.

Nature cell biology·2026
Same author

Cartilage targeting hydrogel nanoplatform degrades BRD4 to alleviate osteoarthritis via Nav1.7 axis.

Nature communications·2026
Same author

Optical write-erase chemical memory state in plasmonic nanoarrays.

Chemical science·2025
Same journal

Interfacial Behavior and Adsorption Mechanisms of Sorbitol Polyether Ester Emulsifiers in D-Phase Emulsion Systems: Applications for Spontaneous Emulsification.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Ti<sub>3</sub> Cluster-Doped 2D Goldene Surface for Electronic and Optical Sensing of Oxygen, Nitrogen, and Hydrocarbon-Based Respiratory Biomarkers: A DFT Study.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Condensation of GO<i>x</i> by Restoring Global Protein Fold on Diazo-Enriched SG Surfaces: Reinstallation of FADs Restores Biocatalytic Sustainability in Quantifying Blood Glucose.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Correction to "Protein Encapsulated DNA Metal Nanocluster as Fluorescent Nanoprobe for Detecting Insulin".

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Rational Design of V<sub>2</sub>O<sub>5</sub> Hierarchical Microspheres with Tunable Porosities and Primary Building Blocks for Enhanced Lithium Storage Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Roles of Anion and Cation Doping in g-C<b><sub>3</sub></b>N<b><sub>4</sub></b> as Artificial SEI for Regulating Interfacial Zn<b><sup>2+</sup></b> Deposition in Aqueous Zinc Metal Anodes.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Researchers developed a fast, inexpensive method to pattern fluorescent organic dyes on gold. Human fibroblasts unexpectedly aligned with aromatic patterns, even with cell-resistant poly(ethylene glycol) present.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Microcontact printing is a versatile technique for surface patterning.
  • Fluorescent organic dyes are crucial for bioimaging and diagnostics.
  • Cell-surface interactions are influenced by material properties.

Purpose of the Study:

  • To develop a simple, rapid, and cost-effective method for micropatterning fluorescent organic dyes on gold substrates.
  • To investigate the cellular response of human fibroblasts to patterned surfaces with distinct chemical functionalities.

Main Methods:

  • Utilized microcontact printing to pattern fluorescein and rhodamine dyes on gold surfaces.
  • Employed poly(ethylene glycol) (PEG) linkers for dye immobilization.
  • Created striped and squared micropatterns.

Related Experiment Videos

  • Co-patterned surfaces with methyl-terminated aliphatic molecules.

    • Main Results:

    • Successfully fabricated well-defined micropatterns of fluorescent organic dyes.
    • Observed an unexpected alignment of human fibroblasts along the aromatic dye patterns.
    • Demonstrated that cellular alignment occurred despite the presence of PEG, a known cell-resistant molecule, in fluorescent regions.

    Conclusions:

    • The developed microcontact printing protocol offers a facile route to create functionalized surfaces for cell studies.
    • Human fibroblasts exhibit specific adhesion and alignment behaviors influenced by aromatic patterns, overriding the presence of PEG.
    • This technique holds potential for creating novel substrates for cell guidance and tissue engineering applications.