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Related Experiment Videos

Increased recombination between active tRNA genes.

Matthew J Pratt-Hyatt1, Kevin M Kapadia, Thomas E Wilson

  • 1Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, 48109-0606, USA.

DNA and Cell Biology
|June 24, 2006
PubMed
Summary
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Active transcription of transfer RNA (tRNA) genes in Saccharomyces cerevisiae elevates recombination between these genes, particularly when both are transcribed. This finding contrasts with the suppression of nearby RNA polymerase II transcription by active tRNA genes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Transfer RNA (tRNA) genes are abundant in eukaryotic genomes and often exist in multicopy families.
  • In Saccharomyces cerevisiae, active tRNA gene transcription by RNA polymerase III suppresses nearby RNA polymerase II transcription, partly due to their proximity to the nucleolus.

Purpose of the Study:

  • To investigate whether active tRNA gene transcription influences recombination rates.
  • To determine if recombination between repetitive tRNA genes, which could lead to detrimental gene deletions, is suppressed.
  • To examine the impact of tRNA genes on recombination within a neighboring retrotransposon.

Main Methods:

  • Experimental manipulation of tRNA gene transcription in Saccharomyces cerevisiae.
  • Measurement of recombination frequencies between active tRNA genes.

Related Experiment Videos

  • Analysis of recombination between retrotransposon terminal repeats adjacent to tRNA genes.

    • Main Results:

    • Contrary to expectations, active transcription of tRNA genes did not suppress recombination; instead, it elevated it.
    • Recombination between tRNA genes was significantly increased when both genes were actively transcribed.
    • The study initiated an investigation into the effect of tRNA genes on retrotransposon recombination.

    Conclusions:

    • Active transcription of tRNA genes promotes recombination between them in yeast.
    • The co-transcription of adjacent tRNA genes is a key factor in this elevated recombination.
    • The functional significance of tRNA genes residing near retrotransposons warrants further investigation.