Related Experiment Video
Updated: Aug 7, 2026
10:28
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
Adiponectin promotes endothelial cell differentiation from human peripheral CD14+ monocytes in vitro
Hui Yang1, Rongxin Zhang, Hong Mu
1Molecular Surgeon Research Center, Division of Vascular Surgery and Endovascular Therapy, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
Journal of Cellular and Molecular Medicine
|June 27, 2006
Summary
Adiponectin promotes the differentiation of monocytes into endothelial cells (ECs). This finding suggests adiponectin
Area of Science:
- Immunology
- Cell Biology
- Cardiovascular Research
Background:
- Adiponectin exhibits anti-atherogenic and anti-inflammatory effects.
- Adiponectin is known to stimulate angiogenesis.
- The role of adiponectin in endothelial differentiation is not well understood.
Purpose of the Study:
- To investigate if adiponectin promotes the differentiation of human peripheral blood CD14(+) monocytes into endothelial cells (ECs).
Main Methods:
- Human peripheral blood CD14(+) monocytes were cultured with or without adiponectin (10 microg/ml) for 10 days.
- Flow cytometry was used to analyze endothelial markers (VEGFR-2, eNOS).
- Real-time PCR assessed the mRNA levels of endothelial (eNOS) and smooth muscle cell (SMC) markers (calponin, SMMHC).
Main Results:
- Adiponectin significantly promoted EC morphology formation from CD14(+) monocytes.
- Adiponectin treatment increased the expression of VEGFR-2 and eNOS.
- Adiponectin decreased the expression of SMC markers, calponin and SMMHC.
Conclusions:
- Adiponectin promotes endothelial differentiation from peripheral blood monocytes.
- This process involves morphological changes and altered expression of endothelial and smooth muscle cell markers.
- Adiponectin-induced EC differentiation may aid in vascular healing and angiogenesis.
