Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of multienzyme production by two mixed strains in solid-state fermentation.

Xiu-Juan Wang1, Ji-Gang Bai, Yun-Xiang Liang

  • 1The Key Laboratory of Crop Biology of Shandong, College of Life Science, Shandong Agricultural University, Tai'an 271018, People's Republic of China. baijg@sdau.edu.cn

Applied Microbiology and Biotechnology
|June 28, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Klebsiella oxytoca</i> P620 and <i>Pseudomonas</i> sp. CFA co-inoculation modulates rhizospheric bacterial communities to alleviate the combined stresses of phenolic acids and nitrate in cucumber.

Frontiers in microbiology·2026
Same author

InSe Nanosheet/Ultrathin Si Heterojunction for Filterless, Self-Powered Ultraviolet Photodetection.

The journal of physical chemistry letters·2025
Same author

Changes in knowledge and attitudes towards mental disorders in a Chinese sample population-a web-based approach.

BMC public health·2025
Same author

Global myocardial work parameters measured by the index beat method are comparable to the average of 10 beats in patients during atrial fibrillation.

Frontiers in cardiovascular medicine·2025
Same author

Infective aortitis caused by Corynebacterium striatum in a patient with twice orthotopic heart transplantation.

BMC infectious diseases·2025
Same author

Application of a Pseudomonas strain mitigates the combined stresses of phenolic acids and salt in soil-grown cucumber.

Ecotoxicology and environmental safety·2025

Researchers optimized Aspergillus niger strains F(3) and F(4) for producing multienzyme preparations. The study identified optimal conditions for enzyme production, enhancing dietary supplementation potential.

Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Microbial Fermentation

Background:

  • Dietary supplementation often requires enzymes like cellulase, hemicellulase, glucoamylase, pectinase, and acidic proteinase.
  • Aspergillus niger is a known producer of various industrial enzymes.
  • Optimizing fungal strains and fermentation conditions is crucial for efficient enzyme production.

Purpose of the Study:

  • To screen and select Aspergillus niger strains for multienzyme preparation production.
  • To determine optimal conditions for culturing selected strains for dietary supplementation.
  • To optimize the fermentation medium composition for maximum enzyme yield.

Main Methods:

  • Screening of five Aspergillus niger strains, with F(3) and F(4) selected for further study.

Related Experiment Videos

  • Optimization of mixture proportion, pitching rate, water content, culture duration, and temperature.
  • Monofactorial and orthogonal experiments to determine the ideal medium composition.

    • Main Results:

    • The optimal mixture proportion was 1:4 (F(3) to F(4)) with a pitching rate of 0.3% (W/W).
    • Optimal culture conditions included 30-36 hours of incubation at 30°C in a bran mash with 54.5% (W/W) water.
    • The optimized medium consisted of 80g bran, 20g cottonseed powder, 1g (NH(4))(2)SO(4), and 0.1g KH(2)PO(4), yielding high enzyme activities.

    Conclusions:

    • Selected Aspergillus niger strains F(3) and F(4) can efficiently produce multienzyme preparations.
    • Optimized fermentation parameters and medium composition significantly enhance enzyme yields.
    • The produced multienzyme preparation holds potential for dietary supplementation applications.