Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subnanosecond-resolution phase-resolved fluorescence imaging technique for biomedical applications.

U S Dinish1, C Y Fu, Z X Chao

  • 1School of Mechanical and Aerospace Engineering, Nanyang Technological University, Nanyang Avenue, Singapore 639798. dinish@ntu.edu.sg

Applied Optics
|June 30, 2006
PubMed
Summary

This study introduces a novel phase-resolved fluorescence imaging technique for early cellular abnormality detection. The method effectively separates fluorescence emissions with subnanosecond lifetime differences, improving diagnostic accuracy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

β-Decay Half-Lives of Neutron-Rich Sulfur to Potassium: Evolution of the N=32 and 34 Subshell Closures below Calcium.

Physical review letters·2025
Same author

Precision Mass Measurements around ^{84}Mo Rule Out ZrNb Cycle Formation in the Rapid Proton-Capture Process at Type I X-Ray Bursts.

Physical review letters·2025
Same author

Z=14 Magicity Revealed by the Mass of the Proton Dripline Nucleus ^{22}Si.

Physical review letters·2025
Same author

Pilot study on photoacoustic imaging and confocal Raman spectroscopy-derived biomarkers for assessing structural and physiological skin changes in atopic dermatitis and metabolic diseases.

Biomedical optics express·2025
Same author

[A systematic review and Bayesian network meta-analysis of the effectiveness of non-pharmacological interventions in treating chronic wound pain in patients].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025
Same author

Non-Invasive Depth Profiling of Base Cosmetic Formulations in the Skin Using Handheld Confocal Raman Spectroscopy.

Journal of biophotonics·2025

Area of Science:

  • Biomedical Optics
  • Fluorescence Spectroscopy
  • Cellular Imaging

Background:

  • Cellular abnormalities can be detected by analyzing fluorescence emissions.
  • Differences in fluorescence lifetime, often subnanosecond, characterize these abnormalities.
  • Existing techniques may struggle to resolve closely related fluorescence lifetimes.

Purpose of the Study:

  • To develop a sensitive frequency domain technique for resolving subnanosecond fluorescence lifetime differences.
  • To enhance the early detection of cellular abnormalities using phase-resolved fluorescence imaging.
  • To optimize system configuration for improved signal processing and accuracy.

Main Methods:

  • A phase-resolved fluorescence imaging technique was employed.
  • Even-step phase shifting and homodyne-assisted signal processing were integrated.

Related Experiment Videos

  • Experiments utilized simulated samples with distinct fluorescence lifetimes at identical wavelengths.

    • Main Results:

    • The proposed technique successfully separated fluorescence emissions with subnanosecond lifetime differences.
    • Selective imaging demonstrated the suppression of one fluorescence emission over another.
    • The system effectively eliminated dc offsets, validating its superiority.

    Conclusions:

    • The developed phase-resolved fluorescence imaging technique offers high resolution for lifetime differences.
    • This method shows significant potential for the early detection of cellular abnormalities.
    • Optimized signal processing enhances the reliability of fluorescence-based diagnostics.