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Published on: August 28, 2018
Production of deuterated lutein by Chlorella protothecoides and its detection by mass spectrometric methods
Prakash Bhosale1, Bogdan Serban, Paul S Bernstein
1Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine, 50 North Medical Drive, Moran Eye Center, Salt Lake City, UT 84132, USA.
Abstract:
Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 599. The rapidly growing microalga had much higher levels (58%) of deuterium substitution relative to previously reported (9-15%) natural sources of lutein.
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