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Updated: Aug 7, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

PARP regulates TGF-beta receptor type II expression in estrogen receptor-positive breast cancer cell lines

Julie A Sterling1, Liang Wu, Sunandita S Banerji

  • 1Department of Biochemistry and Cancer Biology, Medical University of Ohio, 3035 Arlington Avenue, Toledo, OH 43614, USA. sterlingj@uthscsa.edu

Anticancer Research
|July 11, 2006
PubMed
Abstract

Insights

Poly(ADP-ribose)polymerase I (PARP) is crucial for regulating the tumor suppressor gene transforming growth factor beta receptor type II (TbetaRII) in estrogen receptor-positive breast cancer. This finding offers potential pathways to re-express TbetaRII and inhibit tumor growth.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Transforming growth factor beta receptor type II (TbetaRII) expression is diminished in estrogen receptor-positive breast cancer, promoting tumor progression.
  • Understanding TbetaRII gene regulation is vital for developing strategies to restore its tumor-suppressive function.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling TbetaRII gene expression in breast cancer cells.
  • To identify proteins that bind to and regulate the TbetaRII promoter.

Main Methods:

  • Utilized 5' promoter deletion constructs to analyze TbetaRII gene regulation.
  • Employed Electrophoretic Mobility Shift Assays (EMSA) to identify DNA-binding proteins.
  • Affinity purification and mass spectrophotometry were used to identify the binding protein.
  • Chromatin immunoprecipitation (ChIP) assays confirmed in vivo promoter interaction.

Main Results:

  • An essential inverted CCAAT box at position -79 was identified within the TbetaRII promoter.
  • Poly(ADP-ribose)polymerase I (PARP) was identified as the protein binding to this critical promoter region.
  • ChIP assays confirmed PARP's interaction with the TbetaRII promoter in living cells.

Conclusions:

  • Poly(ADP-ribose)polymerase I (PARP) plays a significant role in regulating TbetaRII expression.
  • PARP is essential for maintaining TbetaRII expression in estrogen receptor-positive breast cancer cell lines.
  • This study identifies PARP as a key factor in TbetaRII gene regulation, offering therapeutic targets.

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