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Microcontact Printing of Proteins for Cell Biology
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Patterning Hydrophobic Surfaces by Negative Microcontact Printing and Its Applications.

Han Wu1, Liang Wu1, Xiaohu Zhou1

  • 1Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

Small (Weinheim an Der Bergstrasse, Germany)
|August 23, 2018
PubMed
Summary

A new negative microcontact printing method creates hydrophilic polydopamine patterns on hydrophobic surfaces. This technique enables precise patterning for applications like cell arrays and tunable microlens for imaging.

Keywords:
microlens arraysingle cell arraysurface patterning

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Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Hydrophobic surfaces, including perfluorinated materials, present challenges for patterning and cell adhesion.
  • Developing methods for controlled surface modification is crucial for advanced applications in microfluidics and diagnostics.

Purpose of the Study:

  • To develop a negative microcontact printing method for creating hydrophilic polydopamine patterns on hydrophobic surfaces.
  • To demonstrate the utility of this method for forming arrays of liquid droplets and single cells.
  • To fabricate a microlens array with tunable focal length for imaging.

Main Methods:

  • A uniform polydopamine (PDA) thin film was coated onto a hydrophobic surface.
  • An activated polydimethylsiloxane (PDMS) stamp was used to selectively remove PDA from the surface via microcontact printing.
  • The resulting complementary PDA patterns were utilized for droplet and cell patterning.

Main Results:

  • Hydrophilic PDA patterns with micrometer resolution were successfully fabricated on hydrophobic and perfluorinated surfaces.
  • Reliable formation of liquid droplet and single-cell arrays was achieved on these patterned surfaces.
  • A microlens array with tunable focal length was constructed using the droplet array.

Conclusions:

  • The negative microcontact printing method offers a versatile approach for creating functional patterns on challenging surfaces.
  • This technique is promising for high-throughput chemical and biological screening and analysis.
  • The ability to form droplet and cell arrays, as well as microlenses, highlights its broad applicability.